TACE/ADAM17 Antibody (5A1) - BSA Free

Novus Biologicals | Catalog # NBP3-26523

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5A1 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human TACE/ADAM17 [UniProt P78536]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TACE/ADAM17 Antibody (5A1) - BSA Free

TACE/ADAM17 Antibody (5A1)

Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (5A1) [NBP3-26523] -

Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (5A1) [NBP3-26523] - Staining of Hela Cells with TACE/ADAM17 Antibody (5A1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for TACE/ADAM17 Antibody (5A1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TACE/ADAM17

ADAM17 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Gene Symbol

ADAM17

Additional TACE/ADAM17 Products

Product Documents for TACE/ADAM17 Antibody (5A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for TACE/ADAM17 Antibody (5A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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