Talin1 Antibody (TA205) - BSA Free

Novus Biologicals | Catalog # NBP1-21642

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TA205

Format

BSA Free
View all Talin1 Primary Antibodies »

Product Specifications

Immunogen

Human platelet talin

Reactivity Notes

Predicted cross-reactivities: Chicken, Rabbit

Specificity

Immunoblots the 225 kDa talin band on SDS gels of platelets and other cells. It may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. TA205 recognizes an epitope within residues 139-433.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Talin1 Antibody (TA205) - BSA Free

Flow Cytometry: Talin1 Antibody (TA205) - BSA Free [NBP1-21642]

Flow Cytometry: Talin1 Antibody (TA205) - BSA Free [NBP1-21642]

Flow Cytometry: Talin1 Antibody (TA205) [NBP1-21642] - Human peripheral blood platelets stained with mouse anti-human Talin1.

Applications for Talin1 Antibody (TA205) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Flow Cytometry - Membrane permeabilization is required.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Talin1

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.

Alternate Names

KIAA1027, TLN1

Entrez Gene IDs

7094 (Human)

Gene Symbol

TLN1

UniProt

Q9Y490 (Human)

Additional Talin1 Products

Product Documents for Talin1 Antibody (TA205) - BSA Free

Certificate of Analysis

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Product Specific Notices for Talin1 Antibody (TA205) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Talin1 Antibody (TA205) - BSA Free

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Protocols

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