Tankyrase 1 Antibody

Novus Biologicals | Catalog # NBP1-36994

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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Tankyrase 1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence RECOMBINANT corresponding to N-Terminus according to NP_003738.2.

Epitope

N Terminal Region

Specificity

Fusion protein containing 120aa amino acids from N Terminal region (according to NP_003738.2)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Tankyrase 1 Antibody

Western Blot: Tankyrase 1 Antibody [NBP1-36994]

Western Blot: Tankyrase 1 Antibody [NBP1-36994]

Western Blot: Tankyrase 1 Antibody [NBP1-36994] - HEK293 overexpressing TANK1 (lane 2) and TANK2 (lane 4) and probed with EB02508 (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4 degrees C. Detected by chemiluminescence.

Applications for Tankyrase 1 Antibody

Application
Recommended Usage

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing TANK1 a band of approx. 142 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 142 kDa band according to NP_003738.2.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tankyrase 1

Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. TRF1 is a negative regulator of telomere length maintenance. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. During interphase tankyrase co-localizes with TRF1 to telomeres plus nuclear pore complexes whereas during mitosis tankyrase localizes around the pericentriolar matrix of mitotic chromosomes.

Long Name

TRF1-interacting Ankyrin-related ADP-ribose Polymerase

Alternate Names

PARP5A, PARPL, pART5, TANK1, TINF1, TNKS

Entrez Gene IDs

8658 (Human)

Gene Symbol

TNKS

UniProt

NP_003738.2 (Human)

Additional Tankyrase 1 Products

Product Documents for Tankyrase 1 Antibody

Certificate of Analysis

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Product Specific Notices for Tankyrase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tankyrase 1 Antibody

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