TAS1R3 Antibody

Novus Biologicals | Catalog # NB100-98792

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TAS1R3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from mouse TAS1R3 conjugated to blue carrier protein was used as the antigen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAS1R3 Antibody

TAS1R3 Antibody

Western Blot: TAS1R3 Antibody [NB100-98792] -

The mRNA and protein expressions of STR signaling molecules in taste bud organoids. The (A) mRNA and (B,C) protein expressions of T1R2 and T1R3 were lower in the organoids of the HG group than those in the control group. BZYQF significantly reversed the reduction in the expressions of STR signaling molecules in the organoids of the HG group. Control group: N = 4~5; HG group: N = 4; BZYQF group: N = 4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40573235), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TAS1R3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:500
Application Notes
A dilution of 1 :500 is recommended as a starting point. The optimal dilution should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAS1R3

FUNCTION: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.; SUBUNIT: Forms homodimers or heterodimers with TAS1R1 and TAS1R2. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in circumvallate, foliate and fungiform taste papillae as well as in taste buds on the palate. Also expressed in testis. Not expressed in brain, heart, kidney, liver or spleen. The topographic distribution in various taste papillae is different from those of other T1R members.

Alternate Names

T1R3Sweet taste receptor T1R3, taste receptor type 1 member 3, taste receptor, type 1, member 3, TR3

Entrez Gene IDs

83771 (Mouse); 170634 (Rat)

Gene Symbol

TAS1R3

UniProt

Q925D8 (Mouse)

Additional TAS1R3 Products

Product Documents for TAS1R3 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for TAS1R3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TAS1R3 Antibody

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Protocols

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FAQs

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