TAZ/WWTR1 Antibody - BSA Free

Western Blot: TAZ/WWTR1 AntibodyBSA Free [NB600-220]

TAZ-WWTR1-Antibody-Western-Blot-NB600-220-img0005.jpg

Western Blot: TAZ/WWTR1 AntibodyBSA Free [NB600-220]

Western Blot: TAZ/WWTR1 Antibody [NB600-220] - Western blot analysis of TAZ in MDCK cells using NB600-220 at 1:250 dilution.

Western Blot: TAZ/WWTR1 Antibody - BSA Free [NB600-220] -

Western Blot: TAZ/WWTR1 Antibody - BSA Free [NB600-220] - Pharmacologic targeting the beta 1-integrin-FAK-YAP1/TAZ signaling suppresses cancer spheroids & VASP is required for RhoA-mediated YAP1/TAZ dephosphorylation. a Upper, 3D cancer spheroids were harvested for WB for CTGF. VASP knockdown reduced CTGF protein levels in KM12L4 & L3.6 cells. *p < 0.05 by t-test, n = 3 repeats. Lower, cells on Matrigel were treated with PF–228 (3 μM) & collected for WB. PF-228 reduced YAP1/TAZ protein levels. *p < 0.05 by t-test, n = 3 repeats. Samples derived from the same experiment & gels/blots were processed in parallel. b, c PF-228 (3 μM) or Verteporfin (5 μM) reduced the size of L3.6 spheroids on Matrigel. *p < 0.05 by t-test; n > 50 per group. Bar: 100 µm. d HCT116 cells expressing LacZ (control) or RhoAQ63L were seeded on Matrigel to induce cancer spheroids. Overexpression of RhoAQ63L increased the size of cancer spheroids. *p < 0.05 by t-test; n > 50 per group. e Control & VASP knockdown cancer spheroids were harvested for regular WB & Phos-tagTM gel-based WB. RhoAQ63L increased YAP1/TAZ protein levels & YAP1/TAZ dephosphorylation in control cells & these RhoAQ63L effects on YAP1/TAZ were abrogated by VASP knockdown. Densitometry data are shown on the right. *p < 0.05 by ANOVA, n = 3. f Control & VASP knockdown cancer spheroids were harvested for WB using anti-P-YAP(S127) & YAP1. RhoAQ63L reduced the ratio of P-YAP(S127) to YAP1 & this effect of RhoA on YAP1 dephosphorylation was partially reversed by VASP knockdown. *p < 0.05 by ANOVA, n = 3. Samples derived from the same experiment & gels/blots were processed in parallel. Error bar: S.D Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29872721), licensed under a CC-BY license. Not internally tested by Novus Biologicals.