TBC1D4 [p Ser588] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05451

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all TBC1D4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S588 of human TBC1D4 (NP_055647.2). LGSVD

Modification

p Ser588

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBC1D4 [p Ser588] Antibody - Azide and BSA Free

Western Blot: TBC1D4 [p Ser588] AntibodyAzide and BSA Free [NBP3-05451]

Western Blot: TBC1D4 [p Ser588] AntibodyAzide and BSA Free [NBP3-05451]

Western Blot: TBC1D4 [p Ser588] Antibody [NBP3-05451] - Western blot analysis of extracts of HeLa cells, using TBC1D4 antibody (NBP3-05451) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for TBC1D4 [p Ser588] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TBC1D4

AS160, also known as TBC1D4, is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS-160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of Thr642 is also important in mediating TBC1D4 redistribution from low density microsomes to the cytosol in adipocytes.

Alternate Names

Akt substrate of 160 kDa, AS160Acrg embryonic lethality minimal region ortholog, DKFZp779C0666, KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family member 4, TBC1 domain family, member 4

Gene Symbol

TBC1D4

Additional TBC1D4 Products

Product Documents for TBC1D4 [p Ser588] Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TBC1D4 [p Ser588] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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