TBC1D9 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-55320
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human TBC1 domain family, member 9 using the numbering given in entry NP_055945.1 (GeneID 23158).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TBC1D9 Antibody - BSA Free
Western Blot: TBC1D9 Antibody [NB100-55320]
Western Blot: TBC1D9 Antibody [NB100-55320] - Detection of Human TBC1D9/MDR1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TBC1D9/MDR1 antibody NB100-55320 used for IP at 3 mcg/mg lysate. TBC1D9/MDR1 was also immunoprecipitated by rabbit anti-TBC1D9/MDR1 antibody NB100-55321, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation: TBC1D9 Antibody [NB100-55320]
Immunoprecipitation: TBC1D9 Antibody [NB100-55320] - Detection of human TBC1D9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D9 antibody NB100-55320 used for IP at 6 ug per reaction. TBC1D9 was also immunoprecipitated by rabbit anti-TBC1D9 antibody NB100-55321. For blotting immunoprecipitated TBC1D9, NB100-55321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for TBC1D9 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TBC1D9
Alternate Names
KIAA0882TBC1 domain family member 9, MDR1, TBC1 domain family member 9A, TBC1 domain family, member 9, TBC1 domain family, member 9 (with GRAM domain), TBC1D9A
Entrez Gene IDs
23158 (Human)
Gene Symbol
TBC1D9
UniProt
Additional TBC1D9 Products
Product Documents for TBC1D9 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for TBC1D9 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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