TCF-3/E2A Antibody (6B8)

Novus Biologicals | Catalog # NBP1-42566

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6B8
View all TCF-3/E2A Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human TCF-3/E2A expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

67.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TCF-3/E2A Antibody (6B8)

Western Blot: TCF-3/E2A Antibody (6B8)BSA Free [NBP1-42566]

Western Blot: TCF-3/E2A Antibody (6B8)BSA Free [NBP1-42566]

Western Blot: TCF-3/E2A Antibody (6B8) [NBP1-42566] - Analysis using TCF3 mouse mAb against A549 (1), A431 (2), Hela (3), PANC-1 (4) and PC-3 (5) cell lysate.
Flow Cytometry: TCF-3/E2A Antibody (6B8) - BSA Free [NBP1-42566]

Flow Cytometry: TCF-3/E2A Antibody (6B8) - BSA Free [NBP1-42566]

Flow Cytometry: TCF-3/E2A Antibody (6B8) [NBP1-42566] - Flow cytometry: analysis of A549 cells using anti-TCF3 mAb (green) and negative control (purple).

Applications for TCF-3/E2A Antibody (6B8)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF-3/E2A

T-cell factor 3 and 4 (TCF3 and TCF4) are transcription factors of the High Mobility Group of DNA binding proteins. They are members of a family of four proteins referred to as LEF/TCF transcription factors (LEF1, TCF1, TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. TCF4 generates transcriptionally active complexes with beta catenin in colon carcinoma cells. TCF3 appears to be expressed independently of TCF4 in the hair follicles, stomach epithelium, and keratinocytes of the skin.

Long Name

Transcription Factor 3/E2-alpha

Alternate Names

bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR

Gene Symbol

TCF3

Additional TCF-3/E2A Products

Product Documents for TCF-3/E2A Antibody (6B8)

Certificate of Analysis

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Product Specific Notices for TCF-3/E2A Antibody (6B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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