TCF4 Antibody (4D4C4) - BSA Free

Novus Biologicals | Catalog # NBP2-61896

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D4C4

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human TCF4 (AA: 518-667) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

71.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TCF4 Antibody (4D4C4) - BSA Free

Western Blot: TCF4 Antibody (4D4C4)BSA Free [NBP2-61896]

Western Blot: TCF4 Antibody (4D4C4)BSA Free [NBP2-61896]

Western Blot: TCF4 Antibody (4D4C4) [NBP2-61896] - Analysis using TCF4 mAb against human TCF4 (AA: 518-667) recombinant protein. (Expected MW is 43 kDa)
Flow Cytometry: TCF4 Antibody (4D4C4) - BSA Free [NBP2-61896]

Flow Cytometry: TCF4 Antibody (4D4C4) - BSA Free [NBP2-61896]

Flow Cytometry: TCF4 Antibody (4D4C4) [NBP2-61896] - Analysis of K562 cells using TCF4 mouse mAb (green) and negative control (red).
Western Blot: TCF4 Antibody (4D4C4)BSA Free [NBP2-61896]

Western Blot: TCF4 Antibody (4D4C4)BSA Free [NBP2-61896]

Western Blot: TCF4 Antibody (4D4C4) [NBP2-61896] - Analysis using TCF4 mAb against HEK293 (1) and TCF4 (AA: 518-667)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: TCF4 Antibody (4D4C4) - BSA Free [NBP2-61896]

ELISA: TCF4 Antibody (4D4C4) - BSA Free [NBP2-61896]

ELISA: TCF4 Antibody (4D4C4) [NBP2-61896] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for TCF4 Antibody (4D4C4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF4

This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. ;

Alternate Names

BHLHB19, bHLHb19E2-2, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, ITF2PTHS, MGC149724, SEF-2, SEF2-1, SEF2-1A, SEF2-1B, SEF2MGC149723, SL3-3 enhancer factor 2, TCF-4, transcription factor 4

Gene Symbol

TCF4

Additional TCF4 Products

Product Documents for TCF4 Antibody (4D4C4) - BSA Free

Certificate of Analysis

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Product Specific Notices for TCF4 Antibody (4D4C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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