TCF4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88633

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Single Cell Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TCF4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQD

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCF4 Antibody - BSA Free

Immunohistochemistry-Frozen: TCF4 Antibody [NBP1-88633]

Immunohistochemistry-Frozen: TCF4 Antibody [NBP1-88633]

Immunohistochemistry-Frozen: TCF4 Antibody [NBP1-88633] - Frozen mouse heart sections. Image submitted by a verified customer review.
TCF4 Antibody

Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -

Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neurons.
TCF4 Antibody

Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -

Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
TCF4 Antibody

Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -

Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human duodenum shows strong nuclear positivity in glandular cells.
TCF4 Antibody

Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -

Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human kidney shows strong nuclear positivity in cells in tubules.
TCF4 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TCF4 Antibody - BSA Free [NBP1-88633]

Chromatin Immunoprecipitation-exo-Seq: TCF4 Antibody - BSA Free [NBP1-88633]

ChIP-Exo-Seq composite graph for Anti-TCF4 (NBP1-88633) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for TCF4 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Frozen

Validated from a verified customer review.

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-88633 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF4

TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Alternate Names

BHLHB19, bHLHb19E2-2, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, ITF2PTHS, MGC149724, SEF-2, SEF2-1, SEF2-1A, SEF2-1B, SEF2MGC149723, SL3-3 enhancer factor 2, TCF-4, transcription factor 4

Entrez Gene IDs

6925 (Human)

Gene Symbol

TCF4

UniProt

P15884 (Human)

Additional TCF4 Products

Product Documents for TCF4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TCF4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TCF4 Antibody - BSA Free

Customer Reviews for TCF4 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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4 Stars
100%
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1 Stars
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Customer Images

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  • TCF4 Antibody
    Name: yidong wang
    Application: Immunohistochemistry
    Sample Tested: Frozen mouse heart sections
    Species: Mouse
    Verified Customer | Posted 05/03/2017
    TCF4 Antibody - BSA Free NBP1-88633

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