TCFL5 Antibody

Novus Biologicals | Catalog # NB100-53800

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TCFL5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KYIQERHGDSLKKE corresponding to internal region according to NP_006593.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TCFL5 Antibody

Western Blot: TCFL5 Antibody [NB100-53800]

Western Blot: TCFL5 Antibody [NB100-53800]

Western Blot: TCFL5 Antibody [NB100-53800] - (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for TCFL5 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human testis lysates (calculated MW of 52.7 kDa band according to NP_006593.2). An additional band of unknown identity was also consistently observed at 28 kDa. This band was greatly reduced by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCFL5

TCFL5 is a new bHLH transcription factor that negatively regulates upstream transcription factor-dependent transcription.

Alternate Names

bHLHe82, CHA, Cha transcription factor, CHAMGC46135, E2BP1, E2BP-1HPV-16 E2 binding protein 1, Figlb, HPV-16 E2-binding protein 1, transcription factor-like 5 (basic helix-loop-helix), transcription factor-like 5 protein

Entrez Gene IDs

10732 (Human); 277353 (Mouse)

Gene Symbol

TCFL5

UniProt

NP_006593.2 (Human)

Additional TCFL5 Products

Product Documents for TCFL5 Antibody

Certificate of Analysis

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Product Specific Notices for TCFL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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