TCL1A Antibody
Novus Biologicals | Catalog # NBP1-86958
![TCL1A Antibody Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]](https://beta-resources.rndsystems.com/images/products/nbp1-86958_rabbit-polyclonal-tcl1a-antibody-immunohistochemistry-paraffin-2122025158365.jpg "Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]")
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Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:AECPTLGEAVTDHPDRLWAWEKFVYLDEKQHAWLPLTIEIKDRLQLRVLLRREDVVLGRPMTPTQIGPSLLPIMWQLYPDGRYRSSDSSFWRLVYHIKIDGVEDMLLE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TCL1A Antibody
Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]
Analysis in human lymph node and cerebral cortex. Corresponding TCL1A RNA-seq data are presented for the same tissues.![TCL1A Antibody Western Blot: BLOC1S1 Antibody [NBP1-86958]](https://beta-resources.rndsystems.com/images/products/nbp1-84060_rabbit-polyclonal-igsf6-dora-antibody-western-blot-2122025156437.jpg "Western Blot: BLOC1S1 Antibody [NBP1-86958]")
Western Blot: BLOC1S1 Antibody [NBP1-86958]
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue
![TCL1A Antibody Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]](https://beta-resources.rndsystems.com/images/products/nbp1-86958_rabbit-polyclonal-tcl1a-antibody-immunohistochemistry-paraffin-21220251583612.jpg "Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]")
Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]
Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.![TCL1A Antibody Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]](https://beta-resources.rndsystems.com/images/products/nbp1-86958_rabbit-polyclonal-tcl1a-antibody-immunohistochemistry-paraffin-21220251571314.jpg "Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]")
Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]
Staining of human kidney shows no positivity in cells in tubules as expected.![TCL1A Antibody Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]](https://beta-resources.rndsystems.com/images/products/nbp1-86958_rabbit-polyclonal-tcl1a-antibody-immunohistochemistry-paraffin-21220251571311.jpg "Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]")
Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]
Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.![TCL1A Antibody Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]](https://beta-resources.rndsystems.com/images/products/nbp1-86958_rabbit-polyclonal-tcl1a-antibody-immunohistochemistry-paraffin-2122025157406.jpg "Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]")
Immunohistochemistry-Paraffin: TCL1A Antibody [NBP1-86958]
Staining of human lymph node shows moderate to strong cytoplasmic positivity in lymphoid cells.Applications for TCL1A Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TCL1A
Long Name
T Cell Leukemia Protein 1A
Alternate Names
TCL1-PEN
Gene Symbol
TCL1A
Additional TCL1A Products
Product Documents for TCL1A Antibody
Product Specific Notices for TCL1A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for TCL1A Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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