TCR V beta 3 Antibody (JOVI.3) [Janelia Fluor® 669]

Novus Biologicals | Catalog # NBP2-50356JF669

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Functional, CyTOF-ready

Label

Janelia Fluor 669

Antibody Source

Monoclonal Mouse IgG2A Clone # JOVI.3
View all TCR V beta 3 Primary Antibodies »

Product Specifications

Immunogen

Thymus, spleen and mesenteric lymph nodes isolated from a mouse transgenic for human Vb3 TcR.

Specificity

The antibody recognises any human T cell receptor that has the vB3 region, staining 50-75% % of peripheral blood T cells and T cell lines.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for TCR V beta 3 Antibody (JOVI.3) [Janelia Fluor® 669]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

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Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TCR V beta 3

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas.

Alternate Names

T cell receptor beta locus, T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster, T-cell receptor, beta cluster, TCRB, TRB

Gene Symbol

TRB

Additional TCR V beta 3 Products

Product Documents for TCR V beta 3 Antibody (JOVI.3) [Janelia Fluor® 669]

Certificate of Analysis

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Product Specific Notices for TCR V beta 3 Antibody (JOVI.3) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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