TEAD1 Antibody (2E7N10)

Name: TEAD1 Antibody (2E7N10)
Brand: Novus Biologicals
SKU: NBP3-16593
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16593

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2E7N10 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TEAD1 (P28347). MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TEAD1 Antibody (2E7N10)

Western Blot: TEF1 Antibody (2E7N10) [NBP3-16593]

Western Blot: TEF1 Antibody (2E7N10) [NBP3-16593] - Western blot analysis of extracts of various cell lines, using TEF1 Rabbit mAb (NBP3-16593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593]

Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593] - Immunoprecipitation analysis of 600ug extracts of Mouse skeletal muscle cells using 3ug TEF1 antibody (NBP3-16593). Western blot was performed from the immunoprecipitate using TEF1 antibody (NBP3-16593) at a dilition of 1:1000.

Chromatin Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593] -

Chromatin Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593] - Analysis of extracts of HepG2 cells, using TEAD1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

N/A-TEF1 Antibody (2E7N10)-

Immunoprecipitation-CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illuminafrom 10⁵ Hep G2 cells with 1 μg TEAD1 Rabbit mAb along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of TEAD1 in representative gene loci (SLC7A5), as shown in figure.

Chromatin Immunoprecipitation: TEAD1 Antibody (2E7N10) [NBP3-16593] -

Chromatin Immunoprecipitation: TEAD1 Antibody (2E7N10) [NBP3-16593] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using TEAD1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Chromatin Immunoprecipitation: TEAD1 Antibody (2E7N10) [TEAD1] -

Chromatin Immunoprecipitation: TEAD1 Antibody (2E7N10) [TEAD1] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using TEAD1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Applications for TEAD1 Antibody (2E7N10)

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5ug antibody for 10ug-15ug of Chromatin

ELISA

Recommended starting concentration is 1 ug/ml

Immunoprecipitation

0.5ug-4ug antibody for 400ug-600ug extracts of whole cells

Western Blot

1:500 - 1:2000

Application Notes

CUT&Tag 10 cells /1 ug

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TEAD1

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

Long Name

Transcriptional enhancer factor TEF-1

Alternate Names

AA, NTEF-1, protein GT-IIC, REF1, TCF13, TCF-13, TEA domain family member 1 (SV40 transcriptional enhancer factor), TEAD1, TEAD-1, TEF-1, transcription factor 13, transcriptional enhancer factor 1, transcriptional enhancer factor TEF-1

Gene Symbol

TEAD1

Additional TEAD1 Products

Product Documents for TEAD1 Antibody (2E7N10)

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Certificate of Analysis

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Product Specific Notices for TEAD1 Antibody (2E7N10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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