Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TetR Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of Escherichia coli TetR protein (between residues 25-75) [UniProt P04483]

Localization

TetR peptide conserved in all strains of gram negative bacteria

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TetR Antibody - BSA Free

Western Blot: TetR AntibodyBSA Free [NB600-234]

Western Blot: TetR AntibodyBSA Free [NB600-234]

Western Blot: TetR Antibody [NB600-234] - Analysis of TetR using 23 kDa recombinant protein. Note the presence of a dimer.

Applications for TetR Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100
Application Notes
This TetR antibody is useful for Western Blot and ELISA.

Reviewed Applications

Read 2 reviews rated 5 using NB600-234 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TetR

Tetracycline repressor TetR (tetracycline repressor protein class B from transposon Tn10) belongs to the Tet/Cam family of bacterial regulators and it controls the expression of Tn10 encoded tetracycline resistance. When tetracycline is not present, TetR recognises its operator sequence, tetO, for repressing tetA expression (resistance gene) and its own expression. Upon entry into the cell, tetracycline binds a pocket within the protein core of TetR and induces a conformational change leading to reorientation of DNA binding heads, which in turn abolishes operator binding for triggering resistance gene expression. TetR repressed tetracycline resistance element is responsible for the maintenance of antibiotic resistance traits in strains of Escherichia coli which confers an advantage in selection to E. coli expressing tetracycline resistance. Moreover, TetR has attracted widespread interest because regulators belonging to this family control the expression of genes which are involved in development, fatty acid metabolism, drug resistance and many other cellular processes.

Alternate Names

pSC101 tetR, Tet Repressor, Tet Repressor Protein, TetR, tetracycline repressor protein

Entrez Gene IDs

4924774 (E. coli)

UniProt

P04483 (E. coli)

Additional TetR Products

Product Documents for TetR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TetR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TetR Antibody - BSA Free

Customer Reviews for TetR Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • TetR Antibody - BSA Free
    Name: Khuynh Nguyen
    Application: Western Blot
    Sample Tested: Pellet
    Species: Bacteria
    Verified Customer | Posted 10/06/2023
    The antibody works well. Primary antibody dilution used: 1:2000
    TetR Antibody - BSA Free NB600-234
  • TetR Antibody
    Name: Ashley Barry
    Application: Western Blot
    Sample Tested: combinant cell lines
    Species: Human
    Verified Customer | Posted 12/01/2016
    Western showing 4 cell lines containing a Tet repressor element (Lanes 1-4) with a negative control cell line (lane 5)
    TetR Antibody - BSA Free NB600-234

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Protocols

View specific protocols for TetR Antibody - BSA Free (NB600-234):

TetR Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

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FAQs

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