TetR Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13727

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TetR Primary Antibodies »

Product Specifications

Immunogen

Reacts with residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for TetR Antibody - BSA Free

Western Blot: TetR Antibody [NBP3-13727]

Western Blot: TetR Antibody [NBP3-13727]

Western Blot: TetR Antibody [NBP3-13727] - Detection of Tet- Repressor (TetR) using anti-TetR (NBP3-13727) at a 1:1000 dilution. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
TetR Antibody

ELISA: TetR Antibody [NBP3-13727] -

ELISA: TetR Antibody [NBP3-13727] - Indirect ELISA analysis performed by coating plate with recombinant TetR protein (162.21-2.53 nM). Coated protein was probed with TetR antibody (NBP3-13727) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody.
TetR Antibody - BSA Free

Western Blot: TetR Antibody [NBP3-13727] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Tetracyclin repressor (tetR) antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for TetR Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:10000

Western Blot

1:5000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TetR

TetR, Tet repressor protein, is a 23kD protein that regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.

Alternate Names

pSC101 tetR, Tet Repressor, Tet Repressor Protein, TetR, tetracycline repressor protein

Additional TetR Products

Product Documents for TetR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TetR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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