TFEB Antibody (BLR070G)

Novus Biologicals | Catalog # NBP2-77360

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR070G
View all TFEB Primary Antibodies »

Product Specifications

Immunogen

Immunogen was a peptide representing a region between residue 426 and the C-terminus (residue 476) of human Transcription factor EB using the numbering given in entry NP_009093.1 (Gene ID 7942).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFEB Antibody (BLR070G)

Western Blot: TFEB Antibody (BLR070G) [NBP2-77360]

Western Blot: TFEB Antibody (BLR070G) [NBP2-77360]

Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1,and BW5147.3 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-TFEB recombinant monoclonalantibody [BLR070G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
Western Blot: TFEB Antibody (BLR070G) [NBP2-77360]

Western Blot: TFEB Antibody (BLR070G) [NBP2-77360]

Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from HeLa, Hep-G2, MCF-7, Jurkat, andK-562 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-TFEB recombinant monoclonal antibody[BLR070G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 3 minutes.Lower Panel: Rabbit anti-Actin recombinant monoclonalantibody [BLR057F].
TFEB Antibody (BLR070G) [NBP2-77360] - Detection of human TFEB by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-TFEB recombinant monoclonal [BLR070G] (NBP2-77360) and rabbit anti-TFEB antibodies (NBP2-12758, other rabbit anti-TFEB antibody).

Applications for TFEB Antibody (BLR070G)

Application
Recommended Usage

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: TFEB

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Long Name

Transcription Factor EB

Alternate Names

ALPHATFEB, BHLHE35, TCFEB

Gene Symbol

TFEB

Additional TFEB Products

Product Documents for TFEB Antibody (BLR070G)

Certificate of Analysis

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Product Specific Notices for TFEB Antibody (BLR070G)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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