![Western Blot: TFF3 Antibody [NBP3-13358]](https://resources.rndsystems.com/images/products/TFF3-Antibody-Western-Blot-NBP3-13358-img0001.jpg "Western Blot: TFF3 Antibody [NBP3-13358]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human TFF3. The exact sequence is proprietary.
Reactivity Notes
Mouse (80%), Rat (80%), Pig (88%), Bovine (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for TFF3 Antibody
Western Blot: TFF3 Antibody [NBP3-13358]
Western Blot: TFF3 Antibody [NBP3-13358] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TFF3 antibody (NBP3-13358) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]](https://resources.rndsystems.com/images/products/TFF3-Antibody-Immunohistochemistry-Paraffin-NBP3-13358-img0003.jpg "Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]")
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358] - TFF3 antibody detects secreted TFF3 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. TFF3 stained by TFF3 antibody (NBP3-13358) diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]](https://resources.rndsystems.com/images/products/TFF3-Antibody-Immunohistochemistry-Paraffin-NBP3-13358-img0002.jpg "Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]")
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358] - TFF3 antibody detects TFF3 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. TFF3 stained by TFF3 antibody (NBP3-13358) diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for TFF3 Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TFF3
Long Name
Trefoil Factor 3
Alternate Names
ITF, TFI
Gene Symbol
TFF3
Additional TFF3 Products
Product Documents for TFF3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TFF3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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