Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Functional, In vivo assay, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vivo assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 8D3

Format

Azide Free
View all TfR (Transferrin R) Primary Antibodies »

Product Specifications

Immunogen

Mouse transformed endothelioma cell line t.end1

Marker

Recycling Endosome Marker

Specificity

NB100-64979 recognizes native, soluble and denatured forms of murine CD71. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

Endotoxin Level 0.01 Eu/ug

Scientific Data Images for TfR (Transferrin R) Antibody (8D3) - Azide Free

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979]

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979]

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.
Flow Cytometry: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979]

Flow Cytometry: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979]

Flow Cytometry: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - RPE conjugated rat anti mouse Ter-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse Ter-119 and FITC conjugated rat anti mouse CD71. All experiments performed on mouse bone marrow. Data acquired on the ZE5Cell Analyzer

Applications for TfR (Transferrin R) Antibody (8D3) - Azide Free

Application
Recommended Usage

Flow Cytometry

1:100-1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Immunocytochemistry/Immunofluorescence and In vivo assay were reported in scientific literature.

Reviewed Applications

Read 1 review rated 2 using NB100-64979 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1% Bovine Serum Albumin

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TfR (Transferrin R)

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumor cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anemia and the anemia of chronic disease.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Gene Symbol

TFRC

UniProt

Q62351

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (8D3) - Azide Free

Certificate of Analysis

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Product Specific Notices for TfR (Transferrin R) Antibody (8D3) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TfR (Transferrin R) Antibody (8D3) - Azide Free

Customer Reviews for TfR (Transferrin R) Antibody (8D3) - Azide Free (1)

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Customer Images

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  • TfR (Transferrin R) Antibody (8D3)
    Name: Inês Ribeiro
    Application: Immunocytochemistry
    Sample Tested: bEnd.3 mouse endothelioma cell line
    Species: Mouse
    Verified Customer | Posted 01/14/2022
    mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue).
    0.1 % triton BSA 30 min Primary Ab: 1:50, 1h, RT Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min
    TfR (Transferrin R) Antibody (8D3) - Azide Free NB100-64979
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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