TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-24124
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Kappa Clone # TFRC/9106R
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human fragment (around aa50-250) of human TfR (Transferrin R) protein (exact sequence is proprietary)
Localization
Cell surface
Specificity
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free
Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free [NBP3-24124] -
Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free [NBP3-24124] - Formalin-fixed, paraffin-embedded human placenta stained with TfR (Transferrin R) Antibody (TFRC/9106R). Inset: PBS instead of primary antibody; secondary only negative control.Western Blot: TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free [NBP3-24124] -
Western Blot Analysis of PC-3 cell lysate using TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free.Western Blot: TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free [NBP3-24124] -
Western blot analyses of (1) human lungs, (2) mouse lungs and (3) rat lungs tissue lysates using TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free.Applications for TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: TfR (Transferrin R)
Long Name
Transferrin Receptor
Alternate Names
CD71, TfR (TransferrinR), TFR1, TFRC, TRFR
Gene Symbol
TFRC
Additional TfR (Transferrin R) Products
Product Documents for TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TfR (Transferrin R) Antibody (TFRC/9106R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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