Thimet Oligopeptidase/THOP1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-98355

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Thimet Oligopeptidase/THOP1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse Thimet Oligopeptidase/THOP1 (Accession#: NP_073144.3; Lys 2-Cys687)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Thimet Oligopeptidase/THOP1 Antibody - Azide and BSA Free

Western Blot: Thimet Oligopeptidase/THOP1 Antibody [NBP2-98355]

Western Blot: Thimet Oligopeptidase/THOP1 Antibody [NBP2-98355]

Western Blot: Thimet Oligopeptidase/THOP1 Antibody [NBP2-98355] - Anti-Thimet Oligopeptidase/THOP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HCT116 Whole Cell Lysate. Lane B: HELA Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lane D: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 79 kDa. Observed band size: 72 kDa
Immunoprecipitation: Thimet Oligopeptidase/THOP1 Antibody [NBP2-98355]

Immunoprecipitation: Thimet Oligopeptidase/THOP1 Antibody [NBP2-98355]

Immunoprecipitation: Thimet Oligopeptidase/THOP1 Antibody [NBP2-98355] - Mouse Thimet Oligopeptidase/THOP1 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate 2 ul anti-Mouse Thimet Oligopeptidase/THOP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Thimet Oligopeptidase/THOP1 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 80 kDa

Applications for Thimet Oligopeptidase/THOP1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thimet Oligopeptidase/THOP1

Thimet oligopeptidase, a widely distributed enzyme in most species, plays a role in the metabolism of short neuropeptides (under 20 a.a. residues long). Thimet Oligopeptidase has an influential role in cytoplasmic peptide degradation. Other functions of Thimet Oligopeptidase include the degradation of amyloid precursor protein and the production of amyloidogenic fragments.

Alternate Names

EP24.15

Gene Symbol

THOP1

Additional Thimet Oligopeptidase/THOP1 Products

Product Documents for Thimet Oligopeptidase/THOP1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Thimet Oligopeptidase/THOP1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Thimet Oligopeptidase/THOP1 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Thimet Oligopeptidase/THOP1 Antibody - Azide and BSA Free and earn rewards!

Have you used Thimet Oligopeptidase/THOP1 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies