Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free

Novus Biologicals | Catalog # NBP2-37529

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1H2

Format

BSA Free
View all Thrombopoietin R/Tpo R Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Thrombopoietin R/Tpo R expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

71.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free

Western Blot: Thrombopoietin R/Tpo R Antibody (1H2)BSA Free [NBP2-37529]

Western Blot: Thrombopoietin R/Tpo R Antibody (1H2)BSA Free [NBP2-37529]

Western Blot: Thrombopoietin R/Tpo R Antibody (1H2) [NBP2-37529] - Western blot analysis using MPL mAb against human MPL (AA: 307-362) recombinant protein. (Expected MW is 32.2 kDa)
Flow Cytometry: Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free [NBP2-37529]

Flow Cytometry: Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free [NBP2-37529]

Flow Cytometry: Thrombopoietin R/Tpo R Antibody (1H2) [NBP2-37529] - Flow cytometric analysis of MOLT4 cells using MPL mouse mAb (green) and negative control (red).
ELISA: Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free [NBP2-37529]

ELISA: Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free [NBP2-37529]

ELISA: Thrombopoietin R/Tpo R Antibody (1H2) [NBP2-37529] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thrombopoietin R/Tpo R

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq]

Long Name

Thrombopoietin Receptor

Alternate Names

c-Mpl, CD110, MPL, MPLV, TpoR

Gene Symbol

MPL

Additional Thrombopoietin R/Tpo R Products

Product Documents for Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Thrombopoietin R/Tpo R Antibody (1H2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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