Thyroxine Antibody (T4YCH)
Novus Biologicals | Catalog # NBP1-78604
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Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot, ELISA, Immunoprecipitation, Radioimmunoassay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # T4YCH
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Product Specifications
Immunogen
Thyroxine-BSA
Reactivity Notes
Chemical
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Thyroxine Antibody (T4YCH)
Western Blot: Thyroxine Antibody (T4YCH) [NBP1-78604]
Western Blot: Thyroxine Antibody (T4YCH) [NBP1-78604] - Analysis of L-Thyroxine was performed by loading 600 ng, 300 ng, 150 ng and 75 ng of a Thyroxine-BSA conjugate and 10 uL PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel.ELISA: Thyroxine Antibody (T4YCH) [NBP1-78604]
ELISA: Thyroxine Antibody (T4YCH) [NBP1-78604] - Direct ELISA analysis of L-Thyroxine was performed by coating wells of a 96-well plate with 100 uL per well of L-Thyroxine-bsa conjugated protein diluted in carbonate/bicarbonate buffer.Applications for Thyroxine Antibody (T4YCH)
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunoprecipitation
1:10 - 1:500
Radioimmunoassay
1:100 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS with 1 mg/ml BSA and 30% glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Thyroxine
Alternate Names
T4
Additional Thyroxine Products
Product Documents for Thyroxine Antibody (T4YCH)
Certificate of Analysis
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Product Specific Notices for Thyroxine Antibody (T4YCH)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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