Tie-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-69753

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Tie-2 Primary Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human TIE2 protein (within residues 250-400). [Swiss-Prot Q02763]

Localization

Membrane, single pass type-1 membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tie-2 Antibody - BSA Free

Western Blot: Tie-2 AntibodyBSA Free [NBP1-69753]

Western Blot: Tie-2 AntibodyBSA Free [NBP1-69753]

Western Blot: Tie-2 Antibody [NBP1-69753] - Total protein from human Huvec cells and mouse stomach tissue was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TIE2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: Tie-2 Antibody - BSA Free [NBP1-69753]

Immunohistochemistry-Paraffin: Tie-2 Antibody - BSA Free [NBP1-69753]

Immunohistochemistry-Paraffin: Tie-2 Antibody [NBP1-69753] - Analysis of a FFPE tissue section of mouse stomach using 1:200 dilution of Tie-2 antibody (NBP1-69753). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Western Blot: Tie-2 AntibodyBSA Free [NBP1-69753]

Western Blot: Tie-2 AntibodyBSA Free [NBP1-69753]

Western Blot: Tie-2 Antibody [NBP1-69753] - Detection of TIE2 in Huvec cell lysate
Simple Western: Tie-2 AntibodyBSA Free [NBP1-69753]

Simple Western: Tie-2 AntibodyBSA Free [NBP1-69753]

Simple Western: Tie-2 Antibody [NBP1-69753] - Simple Western lane view shows a specific band for Tie-2 in 0.5 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Tie-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Simple Western

1:50

Western Blot

1:2500
Application Notes
This TIE2 antibody is useful for Western blot where a band is seen ~150 kDa, and Immunohistochemistry-paraffin embedded sections where membrane, cytoplasmic and nuclear signal is seen in mouse intestines. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HUVEC lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 164 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tie-2

TIE2 (tyrosine-protein kinase receptor TIE-2) is an endothelial-selective RTK that is essential to angiopoietin (ANGPT)/TIE2 system which constitutes transmembrane endothelial tyrosine kinase TIE2 and its circulating ligands (ANGPT-1, -2 and ANGPT-3, -4). Activation/phosphorylation state of TIE2 regulates the baseline endothelial quiescence and its response to pathogens as well as cytokines, and TIE2 phosphorylation is largely controlled by the ratio of agonistic ligand ANGPT-1 and competitive inhibitor ANGPT-2. TIE2 exists as homodimer or heterodimer (with TIE1) and interacts with ANGPT1, ANGPT2, ANGPT4, TEK, TNIP2, SHC1, PTPRB, DOK2, GRB2, GRB7, GRB14, PIK3R1 and PTPN11/SHP2 etc for exerting its diverse biological functions. TIE2 regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion, actin cytoskeleton reorganization, and maintenance of vascular quiescence. It exerts anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels, and activates or inhibits angiogenesis, depending on the context (inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts). Targeted deletion of TIE2 or its major agonist ligand, Angpt-1, results in embryonic lethality in mice characterized by defects in blood vessel maturation, lack of recruitment of supporting pericytes and impaired basement membrane formation.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Entrez Gene IDs

7010 (Human)

Gene Symbol

TEK

UniProt

Q02763

Additional Tie-2 Products

Product Documents for Tie-2 Antibody - BSA Free

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Product Specific Notices for Tie-2 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tie-2 Antibody - BSA Free

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Protocols

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