TM4SF18 Antibody - BSA Free

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• TM4SF18 Antibody - BSA Free

  Name: Anonymous

  Application: Immunohistochemistry-Paraffin

  Sample Tested: Multiple tissues

  Species: Human, Canine and Mouse

  Verified Customer | Posted 03/13/2025

  Negative TM4SF18 staining in a canine splenic hemangiosarcoma tumor expressing TM4SF18 on snRNAseq. IHC staining was also negative in multiple human and mouse tissues: kidneys, liver, spleen, lungs...

  Tissue slides were loaded into an automated research stainer, dewaxed and pretreated with EDTA-based epitope retrieval ER2 solution for 20 mins at 100°C. After a peroxide incubation for 5 min, the rabbit antibody against TM4SF18 (NBP2-57543-25UL) (1:50) was incubated for 60 mins at room temperature followed by Bond Polymer (anti-rabbit HRP) incubation for 8 min at room temperature. Mixed DAB reagent (Polymer Refine Detection Kit) was incubated for 10 mins, and Hematoxylin (Refine Detection Kit) counterstaining for 10 mins. After staining, sample slides were washed in water, dehydrated using ethanol gradient (70%, 90%, 100%), washed three times, and mounted in mounting medium.

  
  Bio-Techne Response

  Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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