ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, and the response to oxygen and nutrient deprivation. These functions are regulated by the dimerization of ATF4 with the leucine zipper protein FIAT. ATF4 contains a basic DNA-binding domain (aa 280-300) and a leucine zipper domain (aa 306-334).
TMEM119 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-30792
![Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]](https://resources.rndsystems.com/images/products/TMEM119-Antibody-Immunohistochemistry-Paraffin-NBP2-30792-img0007.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GDGARMVEGRGAEEEEKGSQEGDQEVQGHGVPVETPEAQEEPCSGVLEGAVVAGEGQGELEGSLLLAQEAQGPVGPPESPCACSSVHPS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TMEM119 Antibody - BSA Free (NBP2-30792) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TMEM119 Antibody - BSA Free
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-TMEM119 antibody NBP2-30792 (A) shows similar protein distribution across tissues to independent antibody NBP2-30551 (B).![Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]](https://resources.rndsystems.com/images/products/TMEM119-Antibody-Immunohistochemistry-Paraffin-NBP2-30792-img0008.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792] - Staining of human lymph node strong positivity in reticular fibers in germinal center cells.![Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]](https://resources.rndsystems.com/images/products/TMEM119-Antibody-Immunohistochemistry-Paraffin-NBP2-30792-img0003.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792] - Staining of human cerebral cortex shows moderate to strong membranous positivity in microglia.![Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]](https://resources.rndsystems.com/images/products/TMEM119-Antibody-Immunohistochemistry-Paraffin-NBP2-30792-img0004.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792] - Staining of human liver shows no positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]](https://resources.rndsystems.com/images/products/TMEM119-Antibody-Immunohistochemistry-Paraffin-NBP2-30792-img0006.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792]
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30792] - Staining of human testis shows weak to moderate membranous positivity in peritubular myoid cells.Applications for TMEM119 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TMEM119
Additional TMEM119 Products
Product Documents for TMEM119 Antibody - BSA Free
Product Specific Notices for TMEM119 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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