TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48472

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # TOP1MT/488

Format

IHC-Prediluted
View all TOP1MT Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human TOP1MT protein (Uniprot: Q969P6 )

Localization

Cytoplasmic (Mitochondria)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] - Formalin-fixed, paraffin-embedded human Gallbladder stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/488).
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] - Human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] - Human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted Western Blot: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] -

Western Blot: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] -

Western Blot Analysis of Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted.
TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] -

Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] -

Flow Cytometric Analysis of PFA-fixed MCF-7 cells. TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] -

Immunocytochemistry/ Immunofluorescence: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] -

Immunofluorescent analysis of PFA-fixed MCF-7 cells. TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (green); nuclear counterstain (RedDot).

Applications for TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TOP1MT

The TOP1MT gene encodes a mitochondrial DNA topoisomerase, an enzyme that controls and alters the topologic states of DNAduring transcription. This enzyme catalyzes the transient breaking and rejoining of DNA which allows the strands topass through one another

Alternate Names

mitochondrial, mitochondrial DNA topoisomerase I, topoisomerase (DNA) I, mitochondrial

Gene Symbol

TOP1MT

Additional TOP1MT Products

Product Documents for TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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