TOP1MT Antibody (TOP1MT/488)
Novus Biologicals | Catalog # NBP2-44360
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunofluorescence, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # TOP1MT/488
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Product Specifications
Immunogen
Recombinant full-length human TOP1MT protein (Uniprot: Q969P6 )
Localization
Cytoplasmic (Mitochondria)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47793)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for TOP1MT Antibody (TOP1MT/488)
![Western Blot: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]](https://resources.rndsystems.com/images/products/TOP1MT-Antibody-TOP1MT-488-Western-Blot-NBP2-44360-img0007.jpg "Western Blot: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]")
Western Blot: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]
Western Blot: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] - Analysis of human Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488).![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]](https://resources.rndsystems.com/images/products/TOP1MT-Antibody-TOP1MT-488-Immunohistochemistry-Paraffin-NBP2-44360-img0003.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]")
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] - Human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).![Flow Cytometry: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]](https://resources.rndsystems.com/images/products/TOP1MT-Antibody-TOP1MT-488-Flow-Cytometry-NBP2-44360-img0008.jpg "Flow Cytometry: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]")
Flow Cytometry: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]
Flow Cytometry: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] - Analysis of PFA-fixed MCF-7 cells using TOP1MT Antibody (TOP1MT/488).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]](https://resources.rndsystems.com/images/products/TOP1MT-Antibody-TOP1MT-488-Immunohistochemistry-Paraffin-NBP2-44360-img0002.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]")
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] - Human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).![TOP1MT Antibody (TOP1MT/488) Immunocytochemistry/ Immunofluorescence: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] -](https://resources.rndsystems.com/images/products/nbp2-44360_mouse-monoclonal-top1mt-antibody-top1mt-488-295202516372928.jpg "Immunocytochemistry/ Immunofluorescence: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] -")
Immunocytochemistry/ Immunofluorescence: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] -
Immunofluorescent analysis of PFA-fixed MCF-7 cells. TOP1MT Antibody (TOP1MT/488) followed by goat anti-mouse IgG-CF488 (green); nuclear counterstain (RedDot).Applications for TOP1MT Antibody (TOP1MT/488)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
0.5-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TOP1MT
Alternate Names
mitochondrial, mitochondrial DNA topoisomerase I, topoisomerase (DNA) I, mitochondrial
Gene Symbol
TOP1MT
UniProt
Additional TOP1MT Products
Product Documents for TOP1MT Antibody (TOP1MT/488)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TOP1MT Antibody (TOP1MT/488)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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