TOP1MT Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-93237
![Western Blot: TOP1MT AntibodyAzide and BSA Free [NBP2-93237]](https://resources.rndsystems.com/images/products/TOP1MT-Antibody-Western-Blot-NBP2-93237-img0001.jpg "Western Blot: TOP1MT AntibodyAzide and BSA Free [NBP2-93237]")
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Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1). RATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TOP1MT Antibody - Azide and BSA Free
Western Blot: TOP1MT AntibodyAzide and BSA Free [NBP2-93237]
Western Blot: TOP1MT Antibody [NBP2-93237] - Analysis of extracts of A-549 cells, using TOP1MT at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time![Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]](https://resources.rndsystems.com/images/products/TOP1MT-Antibody-Immunohistochemistry-Paraffin-NBP2-93237-img0003.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]")
Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]
Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP2-93237] - Immunohistochemistry of paraffin-embedded Rat lung using TOP1MT Rabbit pAb (NBP2-93237) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]](https://resources.rndsystems.com/images/products/TOP1MT-Antibody-Immunohistochemistry-Paraffin-NBP2-93237-img0002.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]")
Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]
Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP2-93237] - Immunohistochemistry of paraffin-embedded Human colon using TOP1MT Rabbit pAb (NBP2-93237) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for TOP1MT Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:200-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TOP1MT
Alternate Names
mitochondrial, mitochondrial DNA topoisomerase I, topoisomerase (DNA) I, mitochondrial
Gene Symbol
TOP1MT
Additional TOP1MT Products
Product Documents for TOP1MT Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TOP1MT Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.govCustomer Reviews for TOP1MT Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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