TOR/mTOR [p Ser2481] Antibody (JE59-62)

Name: TOR/mTOR [p Ser2481] Antibody (JE59-62)
Brand: Novus Biologicals
SKU: NBP3-32779
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-32779

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE59-62 expressed in HEK293

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Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser2481 of human TOR/mTOR. (Uniprot: P42345)

Modification

p Ser 2481

Localization

Endoplasmic reticulum membrane, Golgi apparatus membrane, Mitochondrion outer membrane, Lysosome, Cytoplasm, Nucleus, Microsome membrane, Lysosome membrane, Cytoplasmic vesicle, phagosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

289 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TOR/mTOR [p Ser2481] Antibody (JE59-62) (NBP3-32779) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TOR/mTOR [p Ser2481] Antibody (JE59-62)

Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779]

Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779] - Western blot analysis of TOR/mTOR on different lysates with Rabbit anti-TOR/mTOR antibody (NBP3-32779) at 1/5,000 dilution.

Lane 1: HeLa whole cell lysate
Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32779) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for TOR/mTOR [p Ser2481] Antibody (JE59-62)

Application

Recommended Usage

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TOR

The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase which helps to mediate many cellular processes. Specifically, mTOR is invloved in the cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex, making mTOR antibodies useful tools for a number of important research areas.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Gene Symbol

MTOR

Additional TOR Products

Product Documents for TOR/mTOR [p Ser2481] Antibody (JE59-62)

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Product Specific Notices for TOR/mTOR [p Ser2481] Antibody (JE59-62)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.