Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TP53INP2 Primary Antibodies »

Product Specifications

Immunogen

A genomic peptide made to a C-terminal region of the human TP53INP2 protein (within residues 100-220). [Swiss-Prot Q8IXH6]

Reactivity Notes

Immunogen has 81% identity to rat.

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TP53INP2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TP53INP2 Antibody - BSA Free [NBP1-71704]

Immunocytochemistry/ Immunofluorescence: TP53INP2 Antibody - BSA Free [NBP1-71704]

Immunocytochemistry/Immunofluorescence: TP53INP2 Antibody [NBP1-71704] - TP53INP2 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: TP53INP2 Antibody - BSA Free [NBP1-71704]

Immunohistochemistry: TP53INP2 Antibody - BSA Free [NBP1-71704]

Immunohistochemistry: TP53INP2 Antibody [NBP1-71704] - Staining of TP53INP2 in mouse prostate.

Applications for TP53INP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50
Application Notes
This TP53INP2 antibody is useful for ICC/IF and IHC-P. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-71704 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TP53INP2

Tumor protein p53 inducible nuclear protein 2 is necessary for tumor cell invasive migration. Different splicing events in formation of this protein are key determinants of whether the tumor cell invasive morphology exists, and is probably mediated through a Golgi complex interaction. TP53INP2 also binds the Atg8-like proteins and VMP1 during autophagy, and generates scaffolding for autophagosome membranes. LC3 and LC3R also interact with TP53INP2 through an interaction with the autophagosome transmembrane protein VMP1. Thus, the effect of TP53INP2 on tumor growth seems to be both a direct effect on invasion and an indirect effect of insufficient autophagosome scaffolding and inflammation events in response to tumor formation.

Alternate Names

C20orf110, chromosome 20 open reading frame 110, diabetes and obesity regulated, dJ1181N3.1, DKFZp434B2411, DKFZp434O0827, DOR, FLJ21759, FLJ23500, p53-inducible protein U, PINHPIG-U, tumor protein p53 inducible nuclear protein 2, tumor protein p53-inducible nuclear protein 2

Entrez Gene IDs

58476 (Human); 68728 (Mouse)

Gene Symbol

TP53INP2

UniProt

Q8CFU8 (Mouse)

Additional TP53INP2 Products

Product Documents for TP53INP2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TP53INP2 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TP53INP2 Antibody - BSA Free (1)

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  • Name: jennifer guadagno
    Application: Western Blot
    Sample Tested: Mouse Neuro2A whole cell lysate
    Species: Mouse
    Verified Customer | Posted 10/06/2015
    Lane 1: Non-transfected, Lanes 2, 3= INP2 overexpression
    TP53INP2 Antibody - BSA Free NBP1-71704

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Protocols

View specific protocols for TP53INP2 Antibody - BSA Free (NBP1-71704):

TP53INP2 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

TP53INP2 Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

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