TPD52L2/D54 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-36548
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 156 to 206 of human Tumor Protein D52-like 2 using the numbering given in entry NP_003279.2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TPD52L2/D54 Antibody - BSA Free
Western Blot: TPD52L2/D54 Antibody [NBP2-36548]
Western Blot: TPD52L2/D54 Antibody [NBP2-36548] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TPD52L2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: TPD52L2/D54 Antibody [NBP2-36548]
Immunoprecipitation: TPD52L2/D54 Antibody [NBP2-36548] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated TPD52L2, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for TPD52L2/D54 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:1000 - 1:5000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TPD52L2/D54
Alternate Names
DKFZp686A1765, HCCR-binding protein 2, tumor protein D52-like 2hD54D54, tumor protein D54
Gene Symbol
TPD52L2
UniProt
Additional TPD52L2/D54 Products
Product Documents for TPD52L2/D54 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for TPD52L2/D54 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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