TRAF-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03438

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRAF-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF-2 (NP_066961.2). MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TRAF-2 Antibody - BSA Free (NBP3-03438) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TRAF-2 Antibody - BSA Free

Western Blot: TRAF-2 AntibodyBSA Free [NBP3-03438]

Western Blot: TRAF-2 AntibodyBSA Free [NBP3-03438]

Western Blot: TRAF-2 Antibody [NBP3-03438] - Analysis of extracts of various cell lines, using TRAF-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry-Paraffin: TRAF-2 Antibody - BSA Free [NBP3-03438]

Immunohistochemistry-Paraffin: TRAF-2 Antibody - BSA Free [NBP3-03438]

Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP3-03438] - Immunohistochemistry of paraffin-embedded rat testis using [KO Validated] TRAF-2 Rabbit pAb (NBP3-03438) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: TRAF-2 Antibody - BSA Free [NBP3-03438]

Immunohistochemistry-Paraffin: TRAF-2 Antibody - BSA Free [NBP3-03438]

Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP3-03438] - Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] TRAF-2 Rabbit pAb (NBP3-03438) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: TRAF-2 Antibody - BSA Free [NBP3-03438]

Immunohistochemistry-Paraffin: TRAF-2 Antibody - BSA Free [NBP3-03438]

Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP3-03438] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] TRAF-2 Rabbit pAb (NBP3-03438) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: TRAF-2 Antibody - BSA Free [NBP3-03438]

Immunohistochemistry-Paraffin: TRAF-2 Antibody - BSA Free [NBP3-03438]

Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP3-03438] - Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] TRAF-2 Rabbit pAb (NBP3-03438) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for TRAF-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRAF-2

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. TRAF2 is typically described as a 501 amino acid (aa) protein, GenBank no. NP_066961.2. However, various TRAF2 isoforms have been described. Therefore the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed.

Long Name

TNF Receptor-Associated Factor 2

Alternate Names

TRAF2, TRAP3

Gene Symbol

TRAF2

Additional TRAF-2 Products

Product Documents for TRAF-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAF-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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