Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 75402
View all TRAIL/TNFSF10 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRAIL
Thr95-Gly281
Accession # P50591

Specificity

Detects human TRAIL in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TRAIL/TNFSF10 PE‑conjugated Antibody

Detection of TRAIL/TNFSF10 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs), untreated (A) or treated (B) with rhIFN- a for 48 hours (150 ng/mL), were stained with Mouse Anti-Human TRAIL/TNFSF10 PE-conjugated Monoclonal Antibody (Catalog # FAB687P) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # 3832A). Quadrant markers were set based on control antibody staining (Catalog # IC002P).

Applications for Human TRAIL/TNFSF10 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with IFN- alpha

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAIL/TNFSF10

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Long Name

TNF-related Apoptosis-inducing Ligand

Alternate Names

CD253, TNFSF10

Entrez Gene IDs

8743 (Human); 22035 (Mouse); 246775 (Rat)

Gene Symbol

TNFSF10

UniProt

P50591

Additional TRAIL/TNFSF10 Products

Product Documents for Human TRAIL/TNFSF10 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TRAIL/TNFSF10 PE‑conjugated Antibody

For research use only

Citations for Human TRAIL/TNFSF10 PE‑conjugated Antibody

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