Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 150 ng/mL IFN-alpha for 48 hours were stained with Mouse Anti-Human TRAIL/TNFSF10 APC‑conjugated Monoclonal Antibody (Catalog # FAB687A) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TRAIL is a type II transmembrane protein belonging to the TNF
superfamily and is now designated TNFSF10. TRAIL is active as a
homotrimer and is produced by a variety of cell types in both the
membrane bound form and also as a soluble molecule. It binds to any of
the four TRAIL receptors as well as to Osteoprotegerin.
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