Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 75402
View all TRAIL/TNFSF10 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRAIL
Thr95-Gly281
Accession # P50591

Specificity

Detects human TRAIL in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TRAIL/TNFSF10 Antibody

Detection of TRAIL/TNFSF10 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs), (A) treated with rhIFN- alpha for 48 hours (150 ng/mL), or (B) untreated, were stained with Mouse Anti-Human TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB687) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
TRAIL/TNFSF10 antibody in Human Colon by Immunohistochemistry (IHC-P).

TRAIL/TNFSF10 in Human Colon.

TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human colon using Human TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB687) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of lamina propria cells.. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of TRAIL/TNFSF10 by Western Blot

Detection of TRAIL/TNFSF10 by Western Blot

Sensitivity of Colo205 and mesenchymal stem cells (MSCs) to diabody single-chain TNF-related apoptosis-inducing ligand (Db-scTRAIL) activity. (A) Colo205 cells and (B) MSCs were treated with serial dilutions (titration 1:3) of Db-scTRAIL in the absence (filled squares) or in the presence (filled triangles) of 250 ng/ml of bortezomib (BZB). After 18 h, cell viability was determined using crystal violet staining. Data were normalized using BZB-treated cells or cells treated with normal medium for Db-scTRAIL + BZB or Db-scTRAIL alone, respectively (mean ± SD, n = 3). (C) MSCs were transiently transfected (PEI), and the amount of soluble Db-scTRAIL released in cell culture medium was measured by enzyme-Linked Immunosorbent Assay, every 24 h (mean ± SD, n = 3). (D) After 5 days of transient transfection, Db-scTRAIL secreted in cell medium was purified and analyzed by western blotting using a specific antibody against TRAIL (MSC.Unt: MSC untransfected, MSC.TRAIL: MSC transfected with TRAIL). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28553285), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TRAIL/TNFSF10 by Western Blot

Detection of TRAIL/TNFSF10 by Western Blot

Diabody single-chain TNF-related apoptosis-inducing ligand (Db-scTRAIL) released by mesenchymal stem cell (MSC).TRAIL cell line induces specific apoptotic activity in Colo205. (A) Immunoblotting of purified Db-scTRAIL from MSC untransfected (Unt) and MSC.TRAIL cell culture medium (5 days), using a specific antibody anti TRAIL. Three micrograms of purified Db-scTRAIL were used as positive control (Pos Ctr). (B) The bioactivity of the secreted Db-scTRAIL was tested after 18 h of coculture of MSC lines and Colo205 (ratios 1:5 and 1:50). The cocultures were treated in combination with bortezomib (BZB) (250 ng/ml) and/or TRAIL blocking antibody (1 μg/ml). Cell viability was analyzed using crystal violet staining and data were normalized using Colo205 cells treated with BZB as control (mean ± SD, n = 3). (C) Colo205 cells were seeded in the lower chamber of a transwell (8 × 104 cells). After overnight cultivation, the stable cell lines (Mock.TRAIL and MSC.TRAIL) were seeded in the upper chamber (1.6 × 104 cells) and BZB (250 ng/ml) was added to the medium. After 18 h of treatment, Colo205 were collected, stained with the specific cleaved caspase-3 antibody (Asp 175), and analyzed by flow cytometry (mean ± SD, n = 4). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28553285), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TRAIL/TNFSF10 by Western Blot

Detection of TRAIL/TNFSF10 by Western Blot

TRAIL status and its role in docetaxel-resistant prostate cancer (PCa) cell lines (a) Parental and resistant cells were cultured in the presence or absence of the ABCB1 inhibitor PSC833 for 48 h and total cellular protein extracts were used to assess endogenous TRAIL expression by Western blot. Full Western blot image can be found in Figure S2. Upper panel: representative immunoblot showing levels of TRAIL protein present in whole cell extracts. P150 was used as normalizing control. Lower panel: graphs depicting pooled densitometry measurements of TRAIL levels relative to those of p150 in arbitrary units (AU). Data points are presented as mean ± SEM of triplicate measurements. (b) Cell viability was measured using MTT assay and normalized to an untreated control. LNCaP and C4-2B were treated with increasing doses of sTRAIL for 48 h. (c) LNCaPR and C4-2BR were treated for 48 h with vehicle, PSC833 alone, sTRAIL alone, or a combination of 1 μM PSC833 and increasing doses of sTRAIL. (d) Procaspase-3 and activated caspase-3 expression was assessed by Western blot after a pretreatment with PSC833 alone, sTRAIL alone, and PSC833 combined with sTRAIL. Lower panel: graphs depicting densitometry measurements of Procaspase-3 and activated caspase-3 levels relative to those of p150 in arbitrary units (AU). Full Western blot image can be found in Figure S3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33799432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TRAIL/TNFSF10 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (occipital cortex) and human colon

Reviewed Applications

Read 1 review rated 5 using MAB687 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL/TNFSF10

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Long Name

TNF-related Apoptosis-inducing Ligand

Alternate Names

CD253, TNFSF10

Entrez Gene IDs

8743 (Human); 22035 (Mouse); 246775 (Rat)

Gene Symbol

TNFSF10

UniProt

P50591

Additional TRAIL/TNFSF10 Products

Product Documents for Human TRAIL/TNFSF10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TRAIL/TNFSF10 Antibody

For research use only

Citations for Human TRAIL/TNFSF10 Antibody

Customer Reviews for Human TRAIL/TNFSF10 Antibody (1)

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  • Human TRAIL/TNFSF10 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 09/02/2021
    Human TRAIL/TNFSF10 Antibody MAB687

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