Human TRAIL/TNFSF10 Antibody

Catalog # Availability Size / Price Qty
MAB687
MAB687-SP
Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (1)

Human TRAIL/TNFSF10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRAIL in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 75402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL
Thr95-Gly281
Accession # P50591
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TRAIL/TNFSF10 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs), (A) treated with rhIFN- alpha for 48 hours (150 ng/mL), or (B) untreated, were stained with Mouse Anti-Human TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB687) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry TRAIL/TNFSF10 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

TRAIL/TNFSF10 in Human Colon. TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human colon using Human TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB687) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of lamina propria cells.. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL/TNFSF10

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Long Name
TNF-related Apoptosis-inducing Ligand
Entrez Gene IDs
8743 (Human); 22035 (Mouse); 246775 (Rat)
Alternate Names
Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10

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Citations for Human TRAIL/TNFSF10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Potent antitumoral activity of TRAIL through generation of tumor-targeted single-chain fusion proteins
    Authors: B Schneider, S Münkel, A Krippner-Heidenreich, I Grunwald, W S Wels, H Wajant et al.
    Cell Death & Disease
  2. Upregulation of endogenous TRAIL-elicited apoptosis is essential for metformin-mediated antitumor activity against TNBC and NSCLC
    Authors: Shuang Liu, Erik V. Polsdofer, Lukun Zhou, Sanbao Ruan, Hui Lyu, Defu Hou et al.
    Molecular Therapy - Oncolytics
  3. Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity
    Authors: M Siegemund, N Pollak, O Seifert, K Wahl, K Hanak, A Vogel et al.
    Cell Death & Disease
  4. Molecular Profiling of Docetaxel-Resistant Prostate Cancer Cells Identifies Multiple Mechanisms of Therapeutic Resistance
    Authors: TS Lima, D Iglesias-G, LDO Souza, J Stenvang, DS Lima, MA Røder, K Brasso, JMA Moreira
    Cancers, 2021-03-14;13(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. TRAIL receptor gene editing unveils TRAIL-R1 as a master player of apoptosis induced by TRAIL and ER stress
    Authors: F Dufour, T Rattier, AA Constantin, L Zischler, A Morl‚, H Ben Mabrou, E Humblin, G Jacquemin, E Szegezdi, F Delacote, N Marrakchi, G Guichard, C Pellat-Dec, P Vacher, P Legembre, C Garrido, M Olivier
    Oncotarget, 2017-02-07;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Interferon-alpha stimulates TRAIL expression in human keratinocytes and peripheral blood mononuclear cells: implications for the pathogenesis of cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Ferring-Schmitt S, Bieber T, Tuting T, Wenzel J
    Br. J. Dermatol., 2011-09-15;165(5):1118-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Plasmacytoid dendritic cells express TRAIL and induce CD4+ T-cell apoptosis in HIV-1 viremic patients.
    Authors: Stary G, Klein I, Kohlhofer S, Koszik F, Scherzer T, Mullauer L, Quendler H, Kohrgruber N, Stingl G
    Blood, 2009-08-18;114(18):3854-63.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  8. Conjunctival sac fluid contains elevated levels of soluble TRAIL: implications for the anti-tumoral surveillance of the anterior surface of the eye.
    Authors: Secchiero P, Lamberti G, Corallini F, Melloni E, Guarnotta C, Sebastiani A, Zauli G
    J. Cell. Physiol., 2009-01-01;218(1):199-204.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  9. Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells.
    Authors: Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G
    Stem Cells, 2008-09-04;26(11):2955-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Insulin down-regulates TRAIL expression in vascular smooth muscle cells both in vivo and in vitro.
    Authors: Corallini F, Celeghini C, Rizzardi C, Pandolfi A, Di Silvestre S, Vaccarezza M, Zauli G
    J. Cell. Physiol., 2007-07-01;212(1):89-95.
    Species: Human, Rat
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P, Western Blot
  11. Evidence for a role of TNF-related apoptosis-inducing ligand (TRAIL) in the anemia of myelodysplastic syndromes.
    Authors: Campioni D, Secchiero P, Corallini F, Melloni E, Capitani S, Lanza F, Zauli G
    Am. J. Pathol., 2005-02-01;166(2):557-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces rheumatoid arthritis synovial fibroblast proliferation through mitogen-activated protein kinases and phosphatidylinositol 3-kinase/Akt.
    Authors: Morel J, Audo R, Hahne M, Combe B
    J. Biol. Chem., 2005-01-31;280(16):15709-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human TRAIL/TNFSF10 Antibody

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Human TRAIL/TNFSF10 Antibody
By Anonymous on 09/02/2021
Application: WB Sample Tested: Colon tissue Species: Human