Human TRAIL/TNFSF10 Antibody

(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TRAIL in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 75402
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TRAIL
    Thr95-Gly281
    Accession # P50591
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
TRAIL/TNFSF10 in Human Colon. TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human colon using Human TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB687) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of lamina propria cells.. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL/TNFSF10
TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.
  • Long Name:
    TNF-related Apoptosis-inducing Ligand
  • Entrez Gene IDs:
    8743 (Human); 22035 (Mouse)
  • Alternate Names:
    Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. TRAIL receptor gene editing unveils TRAIL-R1 as a master player of apoptosis induced by TRAIL and ER stress
    Authors: F Dufour, T Rattier, AA Constantin, L Zischler, A Morl‚, H Ben Mabrou, E Humblin, G Jacquemin, E Szegezdi, F Delacote, N Marrakchi, G Guichard, C Pellat-Dec, P Vacher, P Legembre, C Garrido, M Olivier
    Oncotarget, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Interferon-alpha stimulates TRAIL expression in human keratinocytes and peripheral blood mononuclear cells: implications for the pathogenesis of cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Ferring-Schmitt S, Bieber T, Tuting T, Wenzel J
    Br. J. Dermatol., 2011;165(5):1118-23.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Conjunctival sac fluid contains elevated levels of soluble TRAIL: implications for the anti-tumoral surveillance of the anterior surface of the eye.
    Authors: Secchiero P, Lamberti G, Corallini F, Melloni E, Guarnotta C, Sebastiani A, Zauli G
    J. Cell. Physiol., 2009;218(1):199-204.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Plasmacytoid dendritic cells express TRAIL and induce CD4+ T-cell apoptosis in HIV-1 viremic patients.
    Authors: Stary G, Klein I, Kohlhofer S, Koszik F, Scherzer T, Mullauer L, Quendler H, Kohrgruber N, Stingl G
    Blood, 2009;114(18):3854-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells.
    Authors: Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G
    Stem Cells, 2008;26(11):2955-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Insulin down-regulates TRAIL expression in vascular smooth muscle cells both in vivo and in vitro.
    Authors: Corallini F, Celeghini C, Rizzardi C, Pandolfi A, Di Silvestre S, Vaccarezza M, Zauli G
    J. Cell. Physiol., 2007;212(1):89-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Evidence for a role of TNF-related apoptosis-inducing ligand (TRAIL) in the anemia of myelodysplastic syndromes.
    Authors: Campioni D, Secchiero P, Corallini F, Melloni E, Capitani S, Lanza F, Zauli G
    Am. J. Pathol., 2005;166(2):557-63.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces rheumatoid arthritis synovial fibroblast proliferation through mitogen-activated protein kinases and phosphatidylinositol 3-kinase/Akt.
    Authors: Morel J, Audo R, Hahne M, Combe B
    J. Biol. Chem., 2005;280(16):15709-18.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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Isotype Controls
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