Transferrin Antibody (OTI5G2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74583

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5G2

Format

Azide and BSA Free
View all Transferrin Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TF(NP_001054) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

75.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Transferrin Antibody (OTI5G2) - Azide and BSA Free

Western Blot: Transferrin Antibody (OTI5G2)Azide and BSA Free [NBP2-74583]

Western Blot: Transferrin Antibody (OTI5G2)Azide and BSA Free [NBP2-74583]

Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).
Immunocytochemistry/ Immunofluorescence: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583]

Immunocytochemistry/ Immunofluorescence: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583]

Immunocytochemistry/Immunofluorescence: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Transferrin.
Flow Cytometry: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583]

Flow Cytometry: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583]

Flow Cytometry: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Transferrin antibody, and then analyzed by flow cytometry.
Western Blot: Transferrin Antibody (OTI5G2)Azide and BSA Free [NBP2-74583]

Western Blot: Transferrin Antibody (OTI5G2)Azide and BSA Free [NBP2-74583]

Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).
Western Blot: Transferrin Antibody (OTI5G2)Azide and BSA Free [NBP2-74583]

Western Blot: Transferrin Antibody (OTI5G2)Azide and BSA Free [NBP2-74583]

Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF.

Applications for Transferrin Antibody (OTI5G2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Transferrin

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Gene Symbol

TF

Additional Transferrin Products

Product Documents for Transferrin Antibody (OTI5G2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Transferrin Antibody (OTI5G2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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