Transglutaminase 3/TGM3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86950

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GSDQERQVFQKALGKLKPNTPFAATSSMGLETEEQEPSIIGKLKVAGMLAVGKEVNLVLLLKNLSRDTKTVTVNMTAWTIIYNGTLVHEVWKDSATMSLDPEEEAEHPIKISYAQYEKYLKSDNMI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Transglutaminase 3/TGM3 Antibody - BSA Free

Transglutaminase 3/TGM3 Antibody

Transglutaminase 3/TGM3 Antibody [NBP1-86950]-Staining of human skeletal muscle shows negative cytoplasmic positivity in myocytes as expected.

Transglutaminase 3/TGM3 Antibody [NBP1-86950]-Staining of human skeletal muscle shows negative cytoplasmic positivity in myocytes as expected.
Transglutaminase 3/TGM3 Antibody - BSA Free Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody - BSA Free [NBP1-86950]

Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody - BSA Free [NBP1-86950]

Staining of human liver shows negative cytoplasmic positivity in hepatocytes.
Transglutaminase 3/TGM3 Antibody - BSA Free Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody - BSA Free [NBP1-86950]

Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody - BSA Free [NBP1-86950]

Staining of human esophagus shows strong cytoplasmic/nucleus positivity in squamous epithelial cells.
Transglutaminase 3/TGM3 Antibody - BSA Free Western Blot: Transglutaminase 3/TGM3 Antibody - BSA Free [NBP1-86950]

Western Blot: Transglutaminase 3/TGM3 Antibody - BSA Free [NBP1-86950]

Analysis in human cell line EFO-21.

Applications for Transglutaminase 3/TGM3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Transglutaminase 3/TGM3

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent. Transglutaminase 3 consists of two polypeptide chains activated from a single precursor protein by proteolysis. Transglutaminase 3 is involved the later stages of cell envelope formation in the epidermis and hair follicle.

Alternate Names

TG3, TgaseE, TGM3

Gene Symbol

TGM3

Additional Transglutaminase 3/TGM3 Products

Product Documents for Transglutaminase 3/TGM3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Transglutaminase 3/TGM3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Transglutaminase 3/TGM3 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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