TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08235

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-TARBP2-1E5

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant full-length human TRBP protein (Uniprot: Q15633)

Localization

Cytoplasm, Cytoplasm > perinuclear region. Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07335).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free [NBP3-08235]

Immunocytochemistry/ Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free [NBP3-08235]

Immunocytochemistry/Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-08235] - Immunofluorescence Analysis of PFA-fixed HeLa cells using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free [NBP3-08235]

Flow Cytometry: TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free [NBP3-08235]

Flow Cytometry: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-08235] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: TRBP Antibody (PCRP-TARBP2-1E5)Azide and BSA Free [NBP3-08235]

Protein Array: TRBP Antibody (PCRP-TARBP2-1E5)Azide and BSA Free [NBP3-08235]

Protein Array: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-08235] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5).

Applications for TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TRBP

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Alternate Names

LOQS, RISC-loading complex subunit TARBP2, TAR (HIV) RNA binding protein 2, TAR (HIV) RNA-binding protein 2, TAR (HIV) RNA-binding protein TRBP1, TAR (HIV-1) RNA binding protein 2, TAR RNA binding protein 2, TAR RNA-binding protein 2, trans-activation responsive RNA-binding protein, Trans-activation-responsive RNA-binding protein, TRBP, TRBP1, TRBP2

Gene Symbol

TARBP2

Additional TRBP Products

Product Documents for TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TRBP Antibody (PCRP-TARBP2-1E5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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