TRHR Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24726

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Canine, Primate

Predicted:

Bovine (100%), Chimpanzee (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A portion of amino acids 350-400 of human TRH-R protein was used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRHR Antibody - BSA Free

Western Blot: TRHR Antibody [NBP2-24726]

Western Blot: TRHR Antibody [NBP2-24726]

Western Blot: TRHR Antibody [NBP2-24726] - Analysis of human TRH-R in human brain lysate in the 1) absence and 2) presence of immunizing peptide at 0.5 ug/ml.
Immunohistochemistry-Paraffin: TRHR Antibody [NBP2-24726]

Immunohistochemistry-Paraffin: TRHR Antibody [NBP2-24726]

Immunohistochemistry-Paraffin: TRHR Antibody [NBP2-24726] - Staining of human thyroid using this antibody at 10 ug/ml.
TRHR Antibody - BSA Free

Western Blot: TRHR Antibody - BSA Free [NBP2-24726] -

TAL enhances the expression of TRHR and DRD2 in SH-SY5Y cells. A, B Immunofluorescence colocalization of DRD2 with TRHR and RAR alpha in SH-SHY5Y cells of the CTR and TAL groups. Scale bar: 25 μm. C, D Analysis of the relative fluorescence density of DRD2 and RAR alpha in cells from the CTR and TAL groups (N = 8/group; error bars represent SEM). Statistical significance: ****P < 0.0001, Student’s t-test. E, F Analysis of the count of colocalized positive cells of DRD2 with TRHR and RAR alpha in different groups of cells (N = 8/group; error bars represent SEM). Statistical significance: ****P < 0.0001, Student’s t-testt. G, H Western blotting analysis of TRHR, P-ERK1/2, ERK1/2, RAR alpha, and DRD2 levels in the SH-SY5Y of CTR and TAL group (N = 8/group; error bars represent SEM). Statistical significance: ****P < 0.0001, Student’s t-test. I, J After knockdown and overexpression of TRHR, Western blotting analysis of TRHR, P-ERK1/2, ERK1/2, RAR alpha, and DRD2 levels in each group of SH-SY5Y cells (N = 8/group; error bars represent SEM). Statistical significance: **P < 0.01, ****P < 0.0001, one-way ANOVA followed by Tukey's test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39736794), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TRHR Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.2% Gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRHR

Receptor for thyrotropin-releasing hormone. This receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.

Long Name

Thyrotropin-releasing Hormone Receptor

Alternate Names

Thyroliberin Receptor

Gene Symbol

TRHR

Additional TRHR Products

Product Documents for TRHR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRHR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRHR Antibody - BSA Free

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Protocols

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FAQs

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