TRIM21/SSA1 Antibody

Novus Biologicals | Catalog # NB100-1209

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRIM21 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CPLNIGSQGSTDY corresponding to C-Terminus according to NP_003132.2.

Localization

Cytoplasmic and nuclear.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRIM21/SSA1 Antibody

Western Blot: TRIM21/SSA1 Antibody [NB100-1209]

Western Blot: TRIM21/SSA1 Antibody [NB100-1209]

Western Blot: TRIM21/SSA1 Antibody [NB100-1209] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B.

Applications for TRIM21/SSA1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.2 - 1 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM21

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is part of the RoSSA ribonucleoprotein which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. The function of the RoSSA particle has not been determined. Two alternatively spliced transcript variants for this gene have been described; however, the full length nature of one variant has not been determined.

Long Name

Tripartite Motif-containing Protein 21

Alternate Names

RNF81, Ro52, SSA1

Entrez Gene IDs

6737 (Human)

Gene Symbol

TRIM21

UniProt

NP_003132. (Human)

Additional TRIM21 Products

Product Documents for TRIM21/SSA1 Antibody

Certificate of Analysis

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Product Specific Notices for TRIM21/SSA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRIM21/SSA1 Antibody

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