TRIM25 Antibody (4Q0D2)

Novus Biologicals | Catalog # NBP3-16382

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4Q0D2 expressed in HEK293
View all TRIM25 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM25 (Q14258). SPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM25 Antibody (4Q0D2)

Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382]

Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382]

Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
TRIM25 Antibody (4Q0D2)

Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] -

Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
TRIM25 Antibody (4Q0D2)

Western Blot: TRIM25 Antibody (4Q0D2) [TRIM25] -

Western Blot: TRIM25 Antibody (4Q0D2) [TRIM25] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Applications for TRIM25 Antibody (4Q0D2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM25

TRIM25 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Long Name

Tripartite Motif-containing Protein 25

Alternate Names

EFP, RNF147, ZNF147

Gene Symbol

TRIM25

Additional TRIM25 Products

Product Documents for TRIM25 Antibody (4Q0D2)

Certificate of Analysis

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Product Specific Notices for TRIM25 Antibody (4Q0D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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