TRIM45 Antibody (OTI3G3) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-74612
![Immunocytochemistry/ Immunofluorescence: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]](https://resources.rndsystems.com/images/products/TRIM45-Antibody-OTI3G3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74612-img0007.jpg "Immunocytochemistry/ Immunofluorescence: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3G3
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 288-580 of human TRIM45(NP_079464) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
64.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRIM45 Antibody (OTI3G3) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]
Immunocytochemistry/Immunofluorescence: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TRIM45.![Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]](https://resources.rndsystems.com/images/products/TRIM45-Antibody-OTI3G3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74612-img0006.jpg "Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]")
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]](https://resources.rndsystems.com/images/products/TRIM45-Antibody-OTI3G3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74612-img0001.jpg "Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]")
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]](https://resources.rndsystems.com/images/products/TRIM45-Antibody-OTI3G3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74612-img0002.jpg "Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]")
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]](https://resources.rndsystems.com/images/products/TRIM45-Antibody-OTI3G3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74612-img0003.jpg "Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]")
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]](https://resources.rndsystems.com/images/products/TRIM45-Antibody-OTI3G3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74612-img0004.jpg "Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]")
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]](https://resources.rndsystems.com/images/products/TRIM45-Antibody-OTI3G3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74612-img0005.jpg "Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]")
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612]
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Applications for TRIM45 Antibody (OTI3G3) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: TRIM45
Alternate Names
RING finger protein 99, RNF99FLJ13181, tripartite motif containing 45, tripartite motif-containing 45, tripartite motif-containing protein 45
Gene Symbol
TRIM45
Additional TRIM45 Products
Product Documents for TRIM45 Antibody (OTI3G3) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TRIM45 Antibody (OTI3G3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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