Rat TrkA ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Kit Contents for Rat TrkA ELISA Kit (Colorimetric)

• ABC diluent buffer
• Antibody diluent buffer
• Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
• Lyophilized recombinant rat TRKA standard
• One 96-well plate precoated with anti- rat TRKA antibody
• Sample diluent buffer
• TMB color developing agent
• TMB stop solution

Preparation and Storage

Background: TrkA

The Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).

Additional TrkA Products

• All Products for TrkA
• TrkA cDNA Clones
• TrkA ELISA Kits
• TrkA Lysates
• TrkA Primary Antibodies
• TrkA Proteins and Enzymes

Product Documents for Rat TrkA ELISA Kit (Colorimetric)