TRMT1L Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88337

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QFSIHASSNVNKQEENGVFIKTTDDTTTDNYIAQGKRKSNEMITNLGKKQKTDVSTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRMT1L Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRMT1L Antibody [NBP1-88337]

Immunocytochemistry/ Immunofluorescence: TRMT1L Antibody [NBP1-88337]

Immunocytochemistry/Immunofluorescence: TRMT1L Antibody [NBP1-88337] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: TRMT1L Antibody [NBP1-88337]

Immunohistochemistry-Paraffin: TRMT1L Antibody [NBP1-88337]

Immunohistochemistry-Paraffin: TRMT1L Antibody [NBP1-88337] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
TRMT1L Antibody - BSA Free Western Blot: TRMT1L Antibody - BSA Free [NBP1-88337]

Western Blot: TRMT1L Antibody - BSA Free [NBP1-88337]

Analysis in control (vector only transfected HEK293T lysate) and TRMT1L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for TRMT1L Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRMT1L

C1orf25 encodes a protein that has some similarity to N2,N2-dimethylguanosine tRNA methyltransferase from other organisms. Studies of the mouse ortholog have shown that this protein plays a role in motor coordination and exploratory behavior, and it may also be involved in modulating postnatal neuronal functions. Alternatively spliced transcripts have been identified for this gene, but their full-length nature is not known.

Alternate Names

C1orf25MST070, chromosome 1 open reading frame 25, MGC25112, MGC57134, TRM1 tRNA methyltransferase 1-like, TRM1LbG120K12.3, TRM1-like protein

Gene Symbol

TRMT1L

Additional TRMT1L Products

Product Documents for TRMT1L Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRMT1L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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