TROP-2 Antibody (001) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89492

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Primate

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001 expressed in HEK293

Format

Azide and BSA Free
View all TROP-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human TROP-2 (Accession#: NP_002344.2; Met 1-Thr 274).

Specificity

Has cross-reactivity in ELISA with: Human E-cadHuman CD146Human CD171Human BCAMHuman ICAM1No cross-reactivity in ELISA with: Human VCAM1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TROP-2 Antibody (001) - Azide and BSA Free

Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492]

Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492]

Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492] - Analysis of human TROP2 expression on MCF-7 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
TROP-2 Antibody (001)

Immunocytochemistry/ Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492] -

Immunocytochemistry/ Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492] - Immunofluorescence staining of TROP-2 in NCI-N87 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89492 (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cell membrane.
TROP-2 Antibody (001) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492] -

Immunocytochemistry/Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492] - Staining of human TROP-2 in MCF-7 cells with NBP2-89492 (1:200). The image showing membrane staining of MCF-7 cells.
TROP-2 Antibody (001) - Azide and BSA Free

Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492] -

Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492] - Flow cytometric analysis of human TROP-2 expression on NCI-N87 cells. Cells were stained with NBP2-89492, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for TROP-2 Antibody (001) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TROP-2

TROP2 encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. [provided by RefSeq]

Long Name

Tumor-associated Calcium Signal Transducer 2

Alternate Names

GA733-1, gp50, T16, TACSTD2, TROP2

Gene Symbol

TACSTD2

Additional TROP-2 Products

Product Documents for TROP-2 Antibody (001) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TROP-2 Antibody (001) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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