Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74639

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8H8

Format

Azide and BSA Free
View all Troponin I Type 1 (slow skeletal) Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TNNI1 (NP_003272) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8)Azide and BSA Free [NBP2-74639]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8)Azide and BSA Free [NBP2-74639]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) - Azide and BSA Free [NBP2-74639] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Troponin I Type 1.
Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) - Azide and BSA Free [NBP2-74639]

Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) - Azide and BSA Free [NBP2-74639]

Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) - Azide and BSA Free [NBP2-74639] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) - Azide and BSA Free [NBP2-74639]

Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) - Azide and BSA Free [NBP2-74639]

Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) - Azide and BSA Free [NBP2-74639] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Troponin I Type 1 (slow skeletal)

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. (provided by RefSeq)

Alternate Names

DKFZp451O223, slow-twitch isoform, SSTNI, TNN1, troponin I type 1 (skeletal, slow), troponin I, skeletal, slow, troponin I, slow skeletal muscle

Gene Symbol

TNNI1

Additional Troponin I Type 1 (slow skeletal) Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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