TRPC5 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94585

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human TRPC5 (NP_036603.1). NLGCKKKTCHGPPLIRTMPRSSGAQGKSKAESSSKRSFMGPSLKKLGLLFSKFNGHMSEPSSEPMYTISDGIVQQHCMWQDIRYSQMEKGKAEACSQSEIN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

111 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPC5 Antibody - Azide and BSA Free

Western Blot: TRPC5 AntibodyAzide and BSA Free [NBP2-94585]

Western Blot: TRPC5 AntibodyAzide and BSA Free [NBP2-94585]

Western Blot: TRPC5 Antibody [NBP2-94585] - Western blot analysis of extracts of various cell lines, using TRPC5 antibody (NBP2-94585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
TRPC5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TRPC5 Antibody - Azide and BSA Free [TRPC5] -

Immunocytochemistry/ Immunofluorescence: TRPC5 Antibody - Azide and BSA Free [TRPC5] - Immunofluorescence analysis of SH-SY5Y cells using TRPC5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
TRPC5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TRPC5 Antibody - Azide and BSA Free [TRPC5] -

Immunocytochemistry/ Immunofluorescence: TRPC5 Antibody - Azide and BSA Free [TRPC5] - Immunofluorescence analysis of Neuro-2a cells using TRPC5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for TRPC5 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRPC5

TRPC5 is expressed in brain and is thought to form a receptor-activated non-selective calcium permeant cation channel. It is likely that the channel is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It may also be activated by intracellular calcium store depletion.

Long Name

Short transient receptor potential channel 5

Alternate Names

hTRP-5, hTRP5, TRP-5, TRP5

Gene Symbol

TRPC5

Additional TRPC5 Products

Product Documents for TRPC5 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRPC5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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