Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRPS1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1000 and 1050 of human Tricho-Rhino-Phalangeal Syndrome Type I using the numbering given in entry AAG21134.1 (GeneID 7227).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TRPS1 Antibody - BSA Free (NBP2-04120) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TRPS1 Antibody - BSA Free

Immunohistochemistry: TRPS1 Antibody [NBP2-04120]

Immunohistochemistry: TRPS1 Antibody [NBP2-04120]

Immunohistochemistry: TRPS1 Antibody [NBP2-04120] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TRPS1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: TRPS1 Antibody [NBP2-04120]

Immunoprecipitation: TRPS1 Antibody [NBP2-04120]

Immunoprecipitation: TRPS1 Antibody [NBP2-04120] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TRPS1 antibody NBP2-04082 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. TRPS1 was also immunoprecipitated by rabbit anti-TRPS1 antibody NBP2-04120, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for TRPS1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

6 µg/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRPS1

The TRPS1 gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcr

Long Name

Tricho-rhino-phalangeal Syndrome Type I Protein

Alternate Names

GC79

Gene Symbol

TRPS1

UniProt

AAG21134.1 (Human)

Additional TRPS1 Products

Product Documents for TRPS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRPS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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