Trypsin 0.25% - EDTA 1mM (1X)

R&D Systems | Catalog # B81350

This item is in process of being discontinued.
R&D Systems
Discontinued Product
B81350 has been discontinued. View all Trypsin products.

Key Product Details

Features

Contains 2.5 gm/L of trypsin (1:250) and 0.38 gm/L EDTA•4Na in Hanks’ Balanced Salt Solution without calcium and magnesium salts.

Applications

Cell Culture

Product Summary for Trypsin 0.25% - EDTA 1mM (1X)

The importance of Trypsin in Cell Culture.
In cell culture research and cell biology studies enzymes are widely used to dissociate tissues and cell monolayers. The goal of cell isolation is to maximize the yield of functionally viable, dissociated cells. Despite the widespread use of enzymes for cell isolation applications over the years, very little is known about their mechanisms of action in cell dissociation and harvesting. Selection of the trypsin enzyme preparation of choice is dependent upon the type cells in culture. Trypsin solutions are enzyme preparations derived from porcine pancreas, generally used for the isolation and dissociation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary for the successful isolation of cells depends primarily on the cell type and the age of the culture.

Product Specifications

Sterility Testing

Pass

Formulation, Preparation, and Storage

Purification

0.2 μm filtered

Shipping

On Dry Ice

Storage

-5 to -20 °C, protected from light

Background: Trypsin

PRSS (Protease; serine) genes produce three isoforms of pancreatic trypsinogen. Based on their electrophoretic mobilities, they are named cationic trypsinogen (trypsin 1/PRSS1), anionic trypsinogen (trypsin 2/PRSS2) and meso- (or in the middle) trypsinogen (trypsin 3/PRSS3). PRSS1 is the predominant form (67%). Following secretion from the pancreas, active trypsin is generated in the duodenum through the interaction of brush-border duodenal enterokinase with trypsinogen.

Alternate Names

TRY2, TRY8, TRYP2

Additional Trypsin Products

Product Documents for Trypsin 0.25% - EDTA 1mM (1X)

Certificate of Analysis

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Related Research Areas

Serine Proteases and Regulators

Citations for Trypsin 0.25% - EDTA 1mM (1X)

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  • Trypsin 0.25% - EDTA 1mM (1X)
    Name: Anonymous
    Verified Customer | Posted 05/04/2021
    I used this to dissociate my MV-1 cells to prepare individual flasks for stimulation
    Trypsin 0.25% - EDTA 1mM (1X) B81350

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