Trypsin 0.25% - EDTA 1mM (1X)

Atlanta Biologicals cell culture products are now sold under the R&D Systems brand
Catalog # Availability Size / Price Qty
B81310
B81350
Trypsin 0.25% - EDTA 1mM (1X)
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Trypsin 0.25% - EDTA 1mM (1X) Summary

Contains 2.5 gm/L of trypsin (1:250) and 0.38 gm/L EDTA•4Na in Hanks’ Balanced Salt Solution without calcium and magnesium salts.

The importance of Trypsin in Cell Culture.

In cell culture research and cell biology studies enzymes are widely used to dissociate tissues and cell monolayers. The goal of cell isolation is to maximize the yield of functionally viable, dissociated cells. Despite the widespread use of enzymes for cell isolation applications over the years, very little is known about their mechanisms of action in cell dissociation and harvesting. Selection of the trypsin enzyme preparation of choice is dependent upon the type cells in culture. Trypsin solutions are enzyme preparations derived from porcine pancreas, generally used for the isolation and dissociation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary for the successful isolation of cells depends primarily on the cell type and the age of the culture.

Specifications

Purification
0.2 μm filtered
Sterility Testing
Pass
Shipping Conditions
On Dry Ice
Storage
-5 to -20 °C, protected from light
Applications
Cell Culture
Species
N/A
Country of Origin
UNITED STATES

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

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Trypsin 0.25% - EDTA 1mM (1X)

Reagents Provided

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